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CosmosID Inc shallow shotgun sequencing
Shallow Shotgun Sequencing, supplied by CosmosID Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shallow+shotgun+sequencing/shallow+shotgun+sequencing/pm40640502-63-12-19
Average 90 stars, based on 1 article reviews
shallow shotgun sequencing - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Shotgun Sequencing:

Article Title: Dysregulated NOX1-NOS2 activity as hallmark of ileitis in mice.
Article Snippet: After homogenization (5x30sec, 5 ms− 1) the upper aqueous layer was collected; this process was repeated and gDNA was precipitated with ethanol and 7.5 M ammonium acetate (Sigma-Aldrich), resuspended in TE buffer (Qiagen) and quantified. .. Shallow shotgun sequencing (3 M Read, 0.5 Gb) for bacteria and ITS 1&2 amplicon sequencing for fungi was performed by CosmosID (Germantown, USA). .. The prepared libraries were sequenced on an Illumina NextSeq 2000 platform (2x150 for bacteria, 2x250 for fungi).

Article Title: Structural and functional characterization of a porcine intestinal microbial ecosystem developed in vitro.
Article Snippet: .. Scientific Reports | (2025) 15:24821 2| https://doi.org/10.1038/s41598-025-10144-5 DNA extraction, library preparation, and sequencing Shallow shotgun sequencing was performed by CosmosID (Germantown, MD) according to a standard protocol as previously described36–38. .. Briefly, DNA was isolated using the QIAGEN DNeasy PowerSoil Pro Kit, according to the manufacturer’s protocol (QIAGEN, Germantown, MD, USA.)

Bacteria:

Article Title: Dysregulated NOX1-NOS2 activity as hallmark of ileitis in mice.
Article Snippet: After homogenization (5x30sec, 5 ms− 1) the upper aqueous layer was collected; this process was repeated and gDNA was precipitated with ethanol and 7.5 M ammonium acetate (Sigma-Aldrich), resuspended in TE buffer (Qiagen) and quantified. .. Shallow shotgun sequencing (3 M Read, 0.5 Gb) for bacteria and ITS 1&2 amplicon sequencing for fungi was performed by CosmosID (Germantown, USA). .. The prepared libraries were sequenced on an Illumina NextSeq 2000 platform (2x150 for bacteria, 2x250 for fungi).

Amplification:

Article Title: Dysregulated NOX1-NOS2 activity as hallmark of ileitis in mice.
Article Snippet: After homogenization (5x30sec, 5 ms− 1) the upper aqueous layer was collected; this process was repeated and gDNA was precipitated with ethanol and 7.5 M ammonium acetate (Sigma-Aldrich), resuspended in TE buffer (Qiagen) and quantified. .. Shallow shotgun sequencing (3 M Read, 0.5 Gb) for bacteria and ITS 1&2 amplicon sequencing for fungi was performed by CosmosID (Germantown, USA). .. The prepared libraries were sequenced on an Illumina NextSeq 2000 platform (2x150 for bacteria, 2x250 for fungi).

Sequencing:

Article Title: Dysregulated NOX1-NOS2 activity as hallmark of ileitis in mice.
Article Snippet: After homogenization (5x30sec, 5 ms− 1) the upper aqueous layer was collected; this process was repeated and gDNA was precipitated with ethanol and 7.5 M ammonium acetate (Sigma-Aldrich), resuspended in TE buffer (Qiagen) and quantified. .. Shallow shotgun sequencing (3 M Read, 0.5 Gb) for bacteria and ITS 1&2 amplicon sequencing for fungi was performed by CosmosID (Germantown, USA). .. The prepared libraries were sequenced on an Illumina NextSeq 2000 platform (2x150 for bacteria, 2x250 for fungi).

Article Title: Structural and functional characterization of a porcine intestinal microbial ecosystem developed in vitro.
Article Snippet: .. Scientific Reports | (2025) 15:24821 2| https://doi.org/10.1038/s41598-025-10144-5 DNA extraction, library preparation, and sequencing Shallow shotgun sequencing was performed by CosmosID (Germantown, MD) according to a standard protocol as previously described36–38. .. Briefly, DNA was isolated using the QIAGEN DNeasy PowerSoil Pro Kit, according to the manufacturer’s protocol (QIAGEN, Germantown, MD, USA.)

DNA Extraction:

Article Title: Structural and functional characterization of a porcine intestinal microbial ecosystem developed in vitro.
Article Snippet: .. Scientific Reports | (2025) 15:24821 2| https://doi.org/10.1038/s41598-025-10144-5 DNA extraction, library preparation, and sequencing Shallow shotgun sequencing was performed by CosmosID (Germantown, MD) according to a standard protocol as previously described36–38. .. Briefly, DNA was isolated using the QIAGEN DNeasy PowerSoil Pro Kit, according to the manufacturer’s protocol (QIAGEN, Germantown, MD, USA.)



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